| 克隆类型 ： |
Mouse Monoclonal Antibody |
|
亚 型 ： |
mouse IgG2b |
|
产品描述 ： |
The CD235ab (HIR2) antibody recognizes a N-terminal homologous portion of glycophorin-A (GPA) and glycophorin-B (GPB), which are single-pass membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities while GPB accounts for S, s and U specificities. GPA and GPB provide the cells with a large mucin-like surface acting as a barrier to cell fusion, therefore minimizing aggregation between red blood cells in the circulation. The CD235ab (HIR2) antibody agglutinates untreated RBC but failed to agglutinate papain-treated cells. This antibody primarly binds to GPA and weakly to GPB. CD235ab is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells. Mature erythrocytes are CD235ab positive whereas CD45 and CD71 negative. |
| 适用物种 ： |
H |
|
应用范围 ： |
E, FC, IHC |
| 应用说明 ： |
Purified antibody is suitable for immunohistochemistry with acetone-fixed frozen and formalin-fixed paraffin sections. E: 1:500-1:1,000; FC: 1:200-1:1,000; IHC: 1:200-1:500 |
| 别 名 ： |
CD235ab; CD235a; Glycophorin-A; PAS-2; Sialoglycoprotein alpha; MN sialoglycoprotein; GYPA; GPA; CD235B; Glycophorin-B; PAS-3; Sialoglycoprotein delta; SS-active sialoglycoprotein; GYPB; GPB |
|
形 态 ： |
Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4 with 0.09% sodium azide. Antibody was purified by Protein-G affinity chromatography. |
| 保存条件 ： |
Store at -20°C. Minimize freeze-thaw cycles. Product is guaranteed one year from the date of shipment. |
|
产品文献 ： |
Shen DC., et al. 1993. J. of Monoclonal Antibody. 9(2): 59; David M., et al. 2002. Leucocyte Typing VII: White Cell Differentiation Antigens. P577-582. |
| 产品链接 ： |
http://www.abbiotec.com/antibodies/cd235ab-hir2-antibody |
| 标 记 物 ： |
|
|
产品说明 ： |
|
