| 克隆类型 ： |
Mouse Monoclonal Antibody |
|
亚 型 ： |
Mouse IgG1 |
|
免 疫 原 ： |
Purified recombinant fragment of BRAF expressed in E. coli. |
|
产品描述 ： |
BRAF (v-raf murine sarcoma viral oncogene homolog B1) is the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway. B-Raf contains three consensus Akt phosphorylation sites (Ser364, Ser428, and Thr439). B-Raf is a key regulatory molecule of the mitogen-activated protein kinase kinase (MEK), it has a long amino-terminal region is essential for homo-dimerization of B-Raf and hetero-dimerization of B-Raf and c-Raf at the plasma membrane, followed by phosphorylation of Thr118 in the amino-terminal B-Raf-specific region. Notably, in calcium ionophore-stimulated HeLa cells, B-Raf could propagate signals to MEK under the basal level of GTP-Ras. Expression of Raf-B is highly restricted with highest levels in the cerebrum and testes. Defects in braf are involved in a wide range of cancers. The BRAF gene mutation is frequently detected in papillary thyroid carcinoma, melanocytic nevi, primary cutaneous melanomas and colorectal cancers.
|
| 适用物种 ： |
H |
|
应用范围 ： |
E, WB, IHC |
| 应用说明 ： |
E: 1:5000-1:10000; WB: 1:500-1:2000; IHC: 1:200-1:1000 |
| 别 名 ： |
RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1; Raf |
|
形 态 ： |
Each vial contains 0.1 ml ascitic fluid with 0.03% sodium azide. |
| 保存条件 ： |
Store at 4°C for short term use only. Store at -20°C for storage over 1 month. Product is guaranteed 6 months from the date of shipment. |
| 产品链接 ： |
http://www.abbiotec.com/antibodies/braf-1f12-antibody |
| 标 记 物 ： |
|
|
产品说明 ： |
|
