NGEC人正常胃黏膜上皮细胞；胃黏膜柔软，活体呈橘红色。胃空虚时形成许多皱襞，充盈时变平坦。幽门处的黏膜形成环形皱襞，突向腔内称幽门瓣。胃黏膜可分为三层，即上皮层(单层柱状上皮)、固有层(由结缔组织构成、含有大量的胃腺)和黏膜肌层(为薄层平滑肌、排列成内环外纵)。NGEC上皮细胞源自胃黏膜的上皮层，细胞为单层柱状上皮，排列整齐，能分泌黏液覆盖于胃黏膜的表面，防止胃酸和胃蛋白酶对胃黏膜的损害。体外培养NGEC人正常胃黏膜上皮细胞为研究细胞功能及致病因子、药物、激素对细胞的损伤、保护和功能调控提供了简单而优越的模型。

 1. 细胞为三代以内，活体。运输过程中细胞出现污染和状态不好，我们免费重新发货。
 2. 实验过程中若确定是客户问题，客户需支付物流和耗材费用，我们可重新发货。其他根据情况灵活处理。
 3. 产品使用过程中，华拓生物可提供技术上的指导。

华拓细胞培养操作指南

细胞培养常见问题分析

1. Dezhi Wang, et al. J Nanobiotechnology. 2024; 22: 479.  PMID: 39134988

Oral administration of Robinia pseudoacacia L. flower exosome-like nanoparticles attenuates gastric and small intestinal mucosal ferroptosis caused by hypoxia through inhibiting HIF-1α- and HIF-2α-mediated lipid peroxidation

2.  Fangting Huang, et al. Infect Immun. 2025 Feb 20;93(3):e00410-24.   PMID: 39976433

Estrogen prevented gingival barrier injury from Porphyromonas gingivalis lipopolysaccharide

3. Zhenmei Song, et al. J Lipid Res. 2025 Aug 12;66(9):100876.    PMID: 40812725
DHA and EPA exacerbate hypoxia-induced ferroptosis in gastric and small intestinal mucosa by disrupting the balance between SLC7A11 upregulation and PUFA-PL accumulation
4. Dezhi Wang, et al. MedComm (2020). 2026 Apr 9;7(4):e70722.  PMID: 41969632   IF=10.7
Ipriflavone From Aquilaria malaccensis Lam. Exosome‐Like Nanoparticles Targets Prolyl Hydroxylase Domain Protein 2 (PHD2) to Enhance Hypoxia‐Inducible Factor‐α (HIF‐α) Hydroxylation Thereby Alleviating Hypoxia‐Induced Gastrointestinal Mucosal Ferroptosis