产品货号Reference:               CAN-RSN-4000
检测方法Kit Type:                  Sandwich ELISA
规格Kit Size:                           96-well break-apart microplate
灵敏度Sensitivity:                  0.004 ng/mL
检测样本Sample Type:          Human Serum / 50 μL
检测时间Total Assay Time:  105 minutes

背景介绍：
抵抗素是一种含有108个氨基酸的12.5 kDa蛋白。它被合成为一种前肽，其疏水性信号肽在分泌之前被裂解。抵抗素在人的血液中以二聚蛋白的形式循环，它由两个92个氨基酸的多肽组成，通过一个二硫键连接在一起。
抵抗素属于类抵抗素激素家族。RELM家族包括RELM-α、RELM-β、RELM-γ和Resistin。RELM-β与抵抗素相关，在结肠表达。在啮齿动物中，抵抗素是由脂肪组织产生的，是葡萄糖代谢和胰岛素敏感性的重要调节因子。啮齿动物的高抵抗性血症导致胰岛素抵抗和易患2型糖尿病。在人类抵抗素是由巨噬细胞产生的，它刺激巨噬细胞分泌促炎症细胞因子。一些研究表明血清抵抗素水平升高与动脉粥样硬化有关。另一项研究显示，有动脉粥样硬化病变的小鼠体内抵抗素水平升高。许多研究试图通过回答在人类肥胖、胰岛素抵抗和/或2型糖尿病中抵抗素水平是否增加的问题来将小鼠数据翻译给人类。一些研究小组未能确定抵抗素水平的变化与肥胖、胰岛素抵抗或2型糖尿病的关系，而另一些使用不同人群和不同检测方法的研究发现，抵抗素水平的变化与一种或多种这类疾病有显著的关系。
基于上述研究，抵抗素可能是代谢性和炎症性疾病的生物标记物和介质。抵抗素生理学的许多领域还有待于研究，以确定它是否可以作为能量代谢和体重调节、代谢综合征、炎症和动脉粥样硬化的标记物。
Resistin is a 12.5 kDa protein containing 108 amino acids. It is synthesised as a pre-peptide and its hydrophobic signal peptide is cleaved before secretion. Resistin circulates in human blood as a dimeric protein consisting of two 92 amino acid polypeptides that are linked by a disulfide bridge.
Resistin belongs to the resistin-like molecule (RELM) hormone family. The RELM family comprises RELM-α, RELM-β, RELM-γ and resistin. RELM-β is related to resistin and is expressed in the colon. In rodents, resistin is produced by adipose tissue and is a significant regulator of glucose metabolism and insulin sensitivity. Hyperresistinemia in rodents causes insulin resistance and predisposition to type 2 diabetes. In humans, resistin is produced by the macrophages, which stimulates the macrophage secretion of pro-inflammatrory cytokines. Some studies have shown a correlation between increased serum resistin levels and atherosclerosis. Another study shows an increase of resistin levels in mice with atherosclerotic lesions. Many studies have tried to translate the mouse data to humans by answering the question whether levels of resistin are increased in human obesity, insulin resistance, and/or type 2 diabetes. Some groups failed to identify changes in resistin levels with obesity, insulin resistance, or type 2 diabetes while other studies that used diverse populations and different assays, have found significant relationships with one or more of these conditions.
Based on the above studies resistin may be a biomarker and a mediator of metabolic and inflammatory diseases. Many areas of resistin physiology remain to be investigated to determine if it can be used as a marker for energy metabolism and body weight regulation, metabolic syndrome, inflammation and atherosclerosis.

注：本产品仅用于科研用途，不能直接用于人体或临床诊断使用。